Genetically Encoded Voltage Indicators
Genetically encoded indicators of voltage offer the promise of less invasive monitoring of identified neuronal activity. Several fluorescent indicators have been developed but the use in in vivo experiments remains minimal. I will discuss efforts by our laboratory to produce indicators that perform better in in vivo experiments in mammalian cortex. This involves novel fluorescent protein discovery and engineering of indicator molecules as well as in vivo application in mammalian brain.
About the Speaker
Vincent Pieribone, Professor, Yale University School of Medicine; Fellow, The John. B. Pierce Laboratory
Our laboratory is focused on the development of novel methods which allow widespread recordings of neuronal electrical activity. Towards this end we focus on three developmental areas: i) Genetically encoded voltage indicators - we use protein engineering and optical microscopy to develop DNA-based fluorescent voltage indicators ii) Miniature, mobile high-speed fluorescent microscopes - for untethered imaging of brain electrical activity in rodents, iii) Identification of novel fluorescent and bioluminescent proteins from marine organisms- for incorporation into in vivo activity indicator molecules. Dr. Pieribone received a B.A. in biology and chemistry from the New York University College of Arts and Sciences in 1986, and a Ph.D. in neuroanatomy and neurophysiology from New York University’s Graduate School of Arts and Sciences in 1992. From 1990 to 1992 he was a National Science Foundation and National Institutes of Health Fogarty International Fellow at the Nobel Institute of Neurophysiology at the Karolinska Institute in Stockholm, Sweden. Dr. Pieribone completed postdoctoral work at The Rockefeller University in New York from 1992 to 1995 with Dr Paul Greengard and became an assistant professor there in 1995. He joined the Pierce Laboratory and Yale University in 1997.